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Microassay for glucose-induced preproinsulin mRNA expression to assess islet functional potency for islet transplantation

BACKGROUND: The capacity for insulin synthesis in islets is important for islet transplantation to succeed. We developed a micro assay that evaluates the potency of human islets by measuring changes in glucose induced insulin gene (INS) expression using a single islet in octuplicate samples. METHODS...

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Bibliografische gegevens
Hoofdauteurs: Omori, Keiko, Mitsuhashi, Masato, Todorov, Ivan, Rawson, Jeffrey, Shiang, Keh-Dong, Kandeel, Fouad, Mullen, Yoko
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2852640/
https://ncbi.nlm.nih.gov/pubmed/20098276
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1097/TP.0b013e3181c4218d
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