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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-deoxy-6-phosphogluconate aldolase from Zymomonas mobilis ZM4

Zymomonas mobilis ZM4 is an organism optimized for ethanol production which uses the Entner–Doudoroff (ED) pathway for the breakdown of glucose. The key enzyme in this process is 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, which produces glyceraldehyde 3-phosphate and pyruvate. In order to pr...

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Bibliographic Details
Main Authors: Ryu, Ho-Chang, Park, Suk-Youl, Lee, Sung Haeng, Kim, Jeong-Sun
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2010
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2852347/
https://ncbi.nlm.nih.gov/pubmed/20383025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110007323
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