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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-deoxy-6-phosphogluconate aldolase from Zymomonas mobilis ZM4

Zymomonas mobilis ZM4 is an organism optimized for ethanol production which uses the Entner–Doudoroff (ED) pathway for the breakdown of glucose. The key enzyme in this process is 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, which produces glyceraldehyde 3-phosphate and pyruvate. In order to pr...

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Détails bibliographiques
Auteurs principaux: Ryu, Ho-Chang, Park, Suk-Youl, Lee, Sung Haeng, Kim, Jeong-Sun
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2010
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2852347/
https://ncbi.nlm.nih.gov/pubmed/20383025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110007323
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