Purification, crystallization and crystallographic analysis of Dictyostelium discoideum phenylalanine hydroxylase in complex with dihydrobiopterin and Fe(III)

Dictyostelium discoideum phenylalanine hydroxylase (DicPAH; residues 1–415) was expressed in Escherichia coli and purified for structural analysis. Apo DicPAH and DicPAH complexed with dihydrobiopterin (BH(2)) and Fe(III) were crystallized using 0.06 M PIPES pH 7.0, 26%(w/v) PEG 2000 by the hanging-...

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Detalhes bibliográficos
Main Authors: Zhuang, Ningning, Seo, Kyung Hey, Chen, Cong, Kim, Hye-Lim, Park, Young Shik, Lee, Kon Ho
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2852345/
https://ncbi.nlm.nih.gov/pubmed/20383023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110007220
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