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Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum

Dihydropyrimidinase (EC 3.5.2.2) is the second enzyme in the reductive pyrimidine-degradation pathway and catalyses the hydrolysis of 5,6-dihydrouracil and 5,6-dihydrothymine to the corresponding N-carbamylated β-amino acids. The recombinant enzyme from the slime mould Dictyostelium discoideum was o...

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Detalhes bibliográficos
Main Authors: Lohkamp, Bernhard, Andersen, Birgit, Piškur, Jure, Dobritzsch, Doreen
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2150923/
https://ncbi.nlm.nih.gov/pubmed/16511257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910503976X
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