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Crystallization and preliminary X-ray crystallographic analysis of a GroEL1 fragment from Mycobacterium tuberculosis H37Rv

Full-length GroEL1 from Mycobacterium tuberculosis H37Rv was cloned, overexpressed and purified. Crystals were obtained by the hanging-drop vapor-diffusion method and contained a 23 kDa GroEL1 fragment. A complete native data set was collected from a single frozen crystal that belonged to the orthor...

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Detalhes bibliográficos
Main Authors: Sielaff, Bernhard, Lee, Ki Seog, Tsai, Francis T. F.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2852334/
https://ncbi.nlm.nih.gov/pubmed/20383012
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110004409
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