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In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy
BACKGROUND: Multicellular tumour sphere models have been shown to closely mimic phenotype characteristics of in vivo solid tumours, or to allow in vitro propagation of cancer stem cells (CSCs). CSCs are usually characterized by the expression of specific membrane markers using flow cytometry (FC) af...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2851689/ https://ncbi.nlm.nih.gov/pubmed/20307308 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2407-10-106 |
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