Release of Alkaline Phosphatase from Cells of Pseudomonas aeruginosa by Manipulation of Cation Concentration and of pH

Pseudomonas aeruginosa ATCC 9027 contains an inducible alkaline phosphatase. The enzyme is readily removed from 14-hr cells by washes in 0.2 m MgCl(2), pH 8.4. Similar washes in tris(hydroxymethyl)aminomethane buffer, 20% sucrose, monovalent ions, or water partially release enzyme from the cells. Th...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Cheng, K.-J., Ingram, J. M., Costerton, J. W.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1970
Schlagworte:	Enzymology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC285053/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4992367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.104.2.748-753.1970
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!