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Release of Alkaline Phosphatase from Cells of Pseudomonas aeruginosa by Manipulation of Cation Concentration and of pH

Pseudomonas aeruginosa ATCC 9027 contains an inducible alkaline phosphatase. The enzyme is readily removed from 14-hr cells by washes in 0.2 m MgCl(2), pH 8.4. Similar washes in tris(hydroxymethyl)aminomethane buffer, 20% sucrose, monovalent ions, or water partially release enzyme from the cells. Th...

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Udgivet i:J Bacteriol
Principais autores: Cheng, K.-J., Ingram, J. M., Costerton, J. W.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1970
Fag:
Enzymology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC285053/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4992367/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.104.2.748-753.1970
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