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Topology of the membrane-bound form of complement protein C9 probed by glycosylation mapping, anti-peptide antibody binding, and disulfide modification
The two N-linked oligosaccharides in native human C9 were deleted by site-specific mutagenesis. This aglycosyl-C9 did not differ from its native form in hemolytic and bactericidal activity. A new N-glycosylation site (K311N/ E313T) was introduced into the turn of a helix-turn-helix [HTH] fold that h...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849895/ https://ncbi.nlm.nih.gov/pubmed/20153530 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molimm.2010.01.013 |
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