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Effective modifications for improved homologous recombination and high-efficiency generation of recombinant adenovirus-based vectors
Generation of adenovirus-based vectors through homologous recombination within Escherichia coli cells is one of the most efficient strategies. A common challenge associated with this method is the formation of colonies containing self-ligated shuttle plasmid. To improve homologous recombination, a n...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2008
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849798/ https://ncbi.nlm.nih.gov/pubmed/18755219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2008.07.024 |
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