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Effective modifications for improved homologous recombination and high-efficiency generation of recombinant adenovirus-based vectors

Generation of adenovirus-based vectors through homologous recombination within Escherichia coli cells is one of the most efficient strategies. A common challenge associated with this method is the formation of colonies containing self-ligated shuttle plasmid. To improve homologous recombination, a n...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wu, Chengxiang, Nerurkar, Vivek R., Yanagihara, Richard, Lu, Yuanan
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849798/
https://ncbi.nlm.nih.gov/pubmed/18755219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2008.07.024
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