Combined Real-Time PCR and rpoB Gene Pyrosequencing for Rapid Identification of Mycobacterium tuberculosis and Determination of Rifampin Resistance Directly in Clinical Specimens

Our laboratory has developed a rapid, sensitive, and specific molecular approach for detection in clinical specimens, within 48 h of receipt, of both Mycobacterium tuberculosis complex (MTBC) DNA and mutations within the 81-bp core region of the rpoB gene that are associated with rifampin (RIF) resi...

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Главные авторы: Halse, Tanya A., Edwards, Justine, Cunningham, Phyllis L., Wolfgang, William J., Dumas, Nellie B., Escuyer, Vincent E., Musser, Kimberlee A.
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology (ASM) 2010
Предметы:
Mycobacteriology and Aerobic Actinomycetes
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849603/
https://ncbi.nlm.nih.gov/pubmed/20107097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02149-09
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