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Development and Application of a PCR-Targeted Gene Disruption Method for Studying CelR Function in Thermobifida fusca

Thermobifida fusca is a high-G+C-content, thermophilic, Gram-positive soil actinobacterium with high cellulolytic activity. In T. fusca, CelR is thought to act as the primary regulator of cellulase gene expression by binding to a 14-bp inverted repeat [5′-(T)GGGAGCGCTCCC(A)] that is upstream of many...

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Bibliografiske detaljer
Main Authors: Deng, Yu, Fong, Stephen S.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849239/
https://ncbi.nlm.nih.gov/pubmed/20097808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02626-09
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