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Development and Application of a PCR-Targeted Gene Disruption Method for Studying CelR Function in Thermobifida fusca
Thermobifida fusca is a high-G+C-content, thermophilic, Gram-positive soil actinobacterium with high cellulolytic activity. In T. fusca, CelR is thought to act as the primary regulator of cellulase gene expression by binding to a 14-bp inverted repeat [5′-(T)GGGAGCGCTCCC(A)] that is upstream of many...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849239/ https://ncbi.nlm.nih.gov/pubmed/20097808 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02626-09 |
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