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Proteolytic Scanning Calorimetry: A Novel Methodology that Probes the Fundamental Features of Protein Kinetic Stability

We introduce proteolytic scanning calorimetry, a modification of the differential scanning calorimetry approach to the determination of protein stability in which a proteolytic enzyme (thermolysin) is used to mimic a harsh environment. This methodology allows the straightforward calculation of the r...

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Hlavní autoři: Tur-Arlandis, Gema, Rodriguez-Larrea, David, Ibarra-Molero, Beatriz, Sanchez-Ruiz, Jose M.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849053/
https://ncbi.nlm.nih.gov/pubmed/20303845
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.11.028
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