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Enrichment and Characterization of Histones by 2D-Hydroxyapatite/Reversed-Phase Liquid Chromatography Mass Spectrometry

Here we report a novel two-dimensional LC-MS method that combines offline hydroxyapatite (HA) chromatography with online reversed-phase liquid chromatography mass spectrometry (HA/RP LC-MS). The 2D-LC-MS method was used to enrich and characterize histones and their posttranslational modifications. T...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Su, Xiaodan, Jacob, Naduparambil K., Amunugama, Ravindra, Hsu, Pang-Hung, Fishel, Richard, Freitas, Michael A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2848711/
https://ncbi.nlm.nih.gov/pubmed/19454230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.01.033
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