Quantification of Rapid Myosin Regulatory Light Chain Phosphorylation Using High-Throughput In-Cell Western Assays: Comparison to Western Immunoblots

BACKGROUND: Quantification of phospho-proteins (PPs) is crucial when studying cellular signaling pathways. Western immunoblotting (WB) is commonly used for the measurement of relative levels of signaling intermediates in experimental samples. However, WB is in general a labour-intensive and low-thro...

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Autores principales: Aguilar, Hector N., Zielnik, Barbara, Tracey, Curtis N., Mitchell, Bryan F.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2010
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2848601/
https://ncbi.nlm.nih.gov/pubmed/20376358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0009965
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