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A novel method for sample preparation of fresh lung cancer tissue for proteomics analysis by tumor cell enrichment and removal of blood contaminants

BACKGROUND: In-depth proteomics analyses of tumors are frequently biased by the presence of blood components and stromal contamination, which leads to large experimental variation and decreases the proteome coverage. We have established a reproducible method to prepare freshly collected lung tumors...

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Detalhes bibliográficos
Main Authors: De Petris, Luigi, Pernemalm, Maria, Elmberger, Göran, Bergman, Per, Orre, Lotta, Lewensohn, Rolf, Lehtiö, Janne
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847553/
https://ncbi.nlm.nih.gov/pubmed/20187940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-8-9
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