Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

BACKGROUND: In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engineeri...

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Bibliographic Details
Main Authors: García-Ruiz, Eva, Maté, Diana, Ballesteros, Antonio, Martinez, Angel T, Alcalde, Miguel
Format: Artigo
Language:Inglês
Published: BioMed Central 2010
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847542/
https://ncbi.nlm.nih.gov/pubmed/20298573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-9-17
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