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RNA isolation method for single embryo transcriptome analysis in zebrafish
BACKGROUND: Transcriptome analysis during embryogenesis usually requires pooling of embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent the average mRNA levels of pooled samples and the biological variation among individuals is confounded. This can irreversibly...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2845602/ https://ncbi.nlm.nih.gov/pubmed/20233395 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-73 |
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