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A Non-Chromatographic Method for the Purification of a Bivalently Active Monoclonal IgG Antibody from Biological Fluids
This paper describes a method for the purification of monoclonal antibodies (rat anti-2,4-dinitrophenyl IgG: IgG(DNP); and mouse anti-digoxin IgG: IgG(Dgn)) from ascites fluid. This procedure (for IgG(DNP)) has three steps: i) precipitation of proteins heavier than immunoglobulins with ammonium sulf...
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Main Authors: | , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2009
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2845436/ https://ncbi.nlm.nih.gov/pubmed/19534466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja9023836 |
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