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A Non-Chromatographic Method for the Purification of a Bivalently Active Monoclonal IgG Antibody from Biological Fluids

This paper describes a method for the purification of monoclonal antibodies (rat anti-2,4-dinitrophenyl IgG: IgG(DNP); and mouse anti-digoxin IgG: IgG(Dgn)) from ascites fluid. This procedure (for IgG(DNP)) has three steps: i) precipitation of proteins heavier than immunoglobulins with ammonium sulf...

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Detalhes bibliográficos
Main Authors: Bilgiçer, Başar, Thomas, Samuel W., Shaw, Bryan F., Kaufman, George K., Krishnamurthy, Vijay M., Estroff, Lara A., Yang, Jerry, Whitesides, George M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2845436/
https://ncbi.nlm.nih.gov/pubmed/19534466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja9023836
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