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Site-directed Mutagenesis of Apolipoprotein CII to Probe the Role of Its Secondary Structure for Activation of Lipoprotein Lipase
Apolipoprotein CII (apoCII) is a necessary activator for lipoprotein lipase (LPL). We had identified four residues (Tyr-63, Ile-66, Asp-69, and Gln-70), presumably contained in an α-helix, as a potential binding site for LPL. We have now used structure prediction, mutagenesis, and functional assays...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2844196/ https://ncbi.nlm.nih.gov/pubmed/20042600 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.022046 |
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