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Role of Pseudomonas aeruginosa outer membrane protein OprH in polymyxin and gentamicin resistance: isolation of an OprH-deficient mutant by gene replacement techniques.

The cloned oprH gene of Pseudomonas aeruginosa was mutated by inserting a 1.4-kbp fragment that encodes tetracycline resistance. This mutated gene was then incorporated into the P. aeruginosa chromosome by homologous recombination. Growth of the resultant oprH::tet mutant in Mg(2+)-deficient medium...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Principais autores: Young, M L, Bains, M, Bell, A, Hancock, R E
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC284378/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1336952/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.36.11.2566
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