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Role of Pseudomonas aeruginosa outer membrane protein OprH in polymyxin and gentamicin resistance: isolation of an OprH-deficient mutant by gene replacement techniques.

The cloned oprH gene of Pseudomonas aeruginosa was mutated by inserting a 1.4-kbp fragment that encodes tetracycline resistance. This mutated gene was then incorporated into the P. aeruginosa chromosome by homologous recombination. Growth of the resultant oprH::tet mutant in Mg(2+)-deficient medium...

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Bibliografiske detaljer
Main Authors: Young, M L, Bains, M, Bell, A, Hancock, R E
Format: Artigo
Sprog:Inglês
Udgivet: 1992
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC284378/
https://ncbi.nlm.nih.gov/pubmed/1336952
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