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Replacing the wild type loxP site in BACs from the public domain with lox66 using a lox66 transposon

BACKGROUND: Chromatin adjoining the site of integration of a transgene affects expression and renders comparisons of closely related transgenes, such as those derived from a BAC deletion series retrofitted with enhancer-traps, unreliable. Gene targeting to a pre-determined site on the chromosome is...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chatterjee, Pradeep K, Shakes, Leighcraft A, Stennett, Naima, Richardson, Vanessa L, Malcolm, Tennison L, Harewood, Ken R
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2841073/
https://ncbi.nlm.nih.gov/pubmed/20170521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-38
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