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Profiling Pancreatic Cancer–Secreted Proteome Using (15)N Amino Acids and Serum-Free Media

OBJECTIVES: A new method of determining protein turnover by labeling protein with (15)N amino acids was used in conjunction with serum-free cell culture to profile secreted proteins that are released by MIA PaCa-2 pancreatic cancer cells in culture. METHODS: MIA PaCa-2 cells were first cultured in D...

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Detalhes bibliográficos
Main Authors: Xiao, Jing, Lee, Wai-Nang Paul, Zhao, Yingchun, Cao, Rui, Go, Vay Liang W., Recker, Robert R., Wang, Qi, Xiao, Gary Guishan
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2835986/
https://ncbi.nlm.nih.gov/pubmed/19904223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1097/MPA.0b013e3181bc44dd
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