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Profiling Pancreatic Cancer–Secreted Proteome Using (15)N Amino Acids and Serum-Free Media
OBJECTIVES: A new method of determining protein turnover by labeling protein with (15)N amino acids was used in conjunction with serum-free cell culture to profile secreted proteins that are released by MIA PaCa-2 pancreatic cancer cells in culture. METHODS: MIA PaCa-2 cells were first cultured in D...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2835986/ https://ncbi.nlm.nih.gov/pubmed/19904223 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1097/MPA.0b013e3181bc44dd |
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