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ENRICHMENT OF CYSTEINYL ADDUCTS OF HUMAN SERUM ALBUMIN
We report a method to enrich cysteinyl adducts of human serum albumin (HSA), representing biomarkers of exposure to systemic electrophiles. Because the major site of HSA adduction is the single free sulfhydryl group at Cys(34), we used thiol-affinity resins to remove mercaptalbumin (i.e., unadducted...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2835821/ https://ncbi.nlm.nih.gov/pubmed/20074543 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.01.006 |
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