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Enzymatic Properties of the Ferredoxin-Dependent Nitrite Reductase from Chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production
The ferredoxin-dependent nitrite reductase from the green alga Chlamydomonas reinhardtii has been cloned, expressed in Escherichia coli as a His-tagged recombinant protein, and purified to homogeneity. The spectra, kinetic properties and substrate-binding parameters of the C. reinhardtii enzyme are...
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| Main Authors: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2833264/ https://ncbi.nlm.nih.gov/pubmed/20039132 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11120-009-9512-5 |
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