Enzymatic Properties of the Ferredoxin-Dependent Nitrite Reductase from Chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production

The ferredoxin-dependent nitrite reductase from the green alga Chlamydomonas reinhardtii has been cloned, expressed in Escherichia coli as a His-tagged recombinant protein, and purified to homogeneity. The spectra, kinetic properties and substrate-binding parameters of the C. reinhardtii enzyme are...

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Main Authors: Hirasawa, Masakazu, Tripathy, Jatindra N., Sommer, Frederik, Somasundaram, Ramasamy, Chung, Jung-Sung, Nestander, Matthew, Kruthiventi, Mahima, Zabet-Moghaddam, Masoud, Johnson, Michael K., Merchant, Sabeeha S., Allen, James P., Knaff, David B.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2833264/
https://ncbi.nlm.nih.gov/pubmed/20039132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11120-009-9512-5
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