Purification of a Specific tRNA by Sepharose-Bound Enzyme
A new procedure for measuring binding of tRNA to aminoacyl-tRNA synthetases is described. The purified isoleucyl-tRNA synthetase from Escherichia coli can be covalently bound to activated Sepharose with retention of approximately 40% of the original enzymatic activity. If crude tRNA is passed throug...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1970
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC283312/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4943171/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.67.2.1057 |
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