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Purification of a Specific tRNA by Sepharose-Bound Enzyme

A new procedure for measuring binding of tRNA to aminoacyl-tRNA synthetases is described. The purified isoleucyl-tRNA synthetase from Escherichia coli can be covalently bound to activated Sepharose with retention of approximately 40% of the original enzymatic activity. If crude tRNA is passed throug...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Denburg, Jeffrey, DeLuca, Marlene
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1970
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC283312/
https://ncbi.nlm.nih.gov/pubmed/4943171
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