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The Full-length Saccharomyces cerevisiae Sgs1 Protein Is a Vigorous DNA Helicase That Preferentially Unwinds Holliday Junctions

The highly conserved RecQ family of DNA helicases has multiple roles in the maintenance of genome stability. Sgs1, the single RecQ homologue in Saccharomyces cerevisiae, acts both early and late during homologous recombination. Here we present the expression, purification, and biochemical analysis o...

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Bibliografski detalji
Glavni autori: Cejka, Petr, Kowalczykowski, Stephen C.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2832980/
https://ncbi.nlm.nih.gov/pubmed/20086270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.083196
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