Rapid Identification and Discrimination of Brucella Isolates by Use of Real-Time PCR and High-Resolution Melt Analysis

Definitive identification of Brucella species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this...

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Bibliographic Details
Main Authors: Winchell, Jonas M., Wolff, Bernard J., Tiller, Rebekah, Bowen, Michael D., Hoffmaster, Alex R.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2010
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2832424/
https://ncbi.nlm.nih.gov/pubmed/20053863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02021-09
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