A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate
A simple and sensitive assay for adenosine 3′:5′-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1970
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC283204/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4318781/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.67.1.305 |
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