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A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate

A simple and sensitive assay for adenosine 3′:5′-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteur principal: Gilman, Alfred G.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1970
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC283204/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4318781/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.67.1.305
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