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Non-natural Amino Acid Fluorophores for One- and Two-Step FRET Applications(†)

Fluorescence resonance energy transfer (FRET) provides a powerful means to study protein conformational changes. However, the incorporation of an exogenous FRET pair into a protein could lead to undesirable structural perturbations of the native fold. One of the viable strategies to minimizing such...

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Autores principales: Rogers, Julie M. G., Lippert, Lisa G., Gai, Feng
Formato: Artigo
Lenguaje:Inglês
Publicado: 2009
Materias:
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2830288/
https://ncbi.nlm.nih.gov/pubmed/20036210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.027
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