PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hl...

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Bibliographic Details
Main Authors: Gerrish, Robert S., Lee, James E., Reed, June, Williams, Joel, Farrell, Larry D., Spiegel, Kathleen M., Sheridan, Peter P., Shields, Malcolm S.
Format: Artigo
Language:Inglês
Published: Centers for Disease Control and Prevention 2007
Subjects:
Dispatch
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2828064/
https://ncbi.nlm.nih.gov/pubmed/17953106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3201/eid1308.060428
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