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Stable DNA unwinding, not "breathing," accounts for single-strand-specific nuclease hypersensitivity of specific A+T-rich sequences.

A long A+T-rich sequence in supercoiled pBR322 DNA is hypersensitive to single-strand-specific nucleases at 37 degrees C but not at reduced temperature. The basis for the nuclease hypersensitivity is stable DNA unwinding as revealed by (i) the same temperature dependence for hypersensitivity and for...

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Sonraí Bibleagrafaíochta
Main Authors: Kowalski, D, Natale, D A, Eddy, M J
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1988
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC282773/
https://ncbi.nlm.nih.gov/pubmed/2849106
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