Improved methods for expression and purification of S. cerevisiae TFIIF and TFIIH; Identification of a functional E.coli promoter and internal translation initiation within the N-terminal coding region of the TFIIF TFG1 subunit

The basal RNA polymerase II (RNAPII) transcription machinery is composed of RNAPII and the general transcription factors (TF) TATA binding protein (TBP), TFIIB, TFIIE, TFIIF and TFIIH. Due to the powerful genetic and molecular approaches that can be utilized, the budding yeast S. cerevisiae has prov...

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Hlavní autoři: Yang, Chen, Khaperskyy, Denys A., Hou, Min, Ponticelli, Alfred S.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2827679/
https://ncbi.nlm.nih.gov/pubmed/19818408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.09.021
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