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Microfluidic Preparative Free-Flow Isoelectric Focusing: System Optimization for Protein Complex Separation

Isoelectric Focusing (IEF) is the first step for two-dimensional (2D) gel electrophoresis and plays an important role in sample purification for proteomics. However, biases in protein size and pI resolution, as well as limitations in sample volume, gel capacity, sample loss, and experimental time, r...

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Detalhes bibliográficos
Main Authors: Wen, Jian, Wilker, Erik W., Yaffe, Michael B., Jensen, Klavs F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2826279/
https://ncbi.nlm.nih.gov/pubmed/20092256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac902157e
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