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Developing bifunctional β-lactamase molecules with built-in target-recognizing module for prodrug therapy: identification of Enterobacter Cloacae P99 cephalosporinase loops suitable for randomization and phage-display selection

This study was focused on developing catalytically active β-lactamase enzyme molecules that have target-recognizing sites built within their scaffold. Using phage-display approach, nine libraries were constructed by inserting the randomized linear or cysteine-constrained heptapeptides in the five di...

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Detalhes bibliográficos
Main Authors: Shukla, Girja S., Krag, David N.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2824592/
https://ncbi.nlm.nih.gov/pubmed/19437416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jmr.957
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