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Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymerase.

The virion-associated RNA polymerase and the structural nucleoprotein of influenza A virus were separated by sodium dodecyl sulfate/PAGE, electroblotted to a polyvinylidine membrane, and eluted with good recovery from the membrane. After renaturation by incubating with Escherichia coli thioredoxin,...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Szewczyk, B, Laver, W G, Summers, D F
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1988
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282313/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3054875/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.21.7907
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