Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymerase.

The virion-associated RNA polymerase and the structural nucleoprotein of influenza A virus were separated by sodium dodecyl sulfate/PAGE, electroblotted to a polyvinylidine membrane, and eluted with good recovery from the membrane. After renaturation by incubating with Escherichia coli thioredoxin,...

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Bibliographic Details
Main Authors: Szewczyk, B, Laver, W G, Summers, D F
Format: Artigo
Language:Inglês
Published: 1988
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC282313/
https://ncbi.nlm.nih.gov/pubmed/3054875
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