Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.

N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us...

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Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Block, M R, Glick, B S, Wilcox, C A, Wieland, F T, Rothman, J E
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282295/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3186695/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.21.7852
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