Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us...
Zapisane w:
| Wydane w: | Proc Natl Acad Sci U S A |
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| Główni autorzy: | , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
National Academy of Sciences
1988
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282295/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3186695/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.21.7852 |
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