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Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.

N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us...

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Sonraí Bibleagrafaíochta
Main Authors: Block, M R, Glick, B S, Wilcox, C A, Wieland, F T, Rothman, J E
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1988
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC282295/
https://ncbi.nlm.nih.gov/pubmed/3186695
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