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Functional reconstitution of a proton-translocating system responsive to fusicoccin.
Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the...
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| Опубликовано в: : | Proc Natl Acad Sci U S A |
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| Главные авторы: | , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1988
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282294/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2903497/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.21.7849 |
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