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Functional reconstitution of a proton-translocating system responsive to fusicoccin.
Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1988
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282294/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2903497/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.21.7849 |
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