Functional reconstitution of a proton-translocating system responsive to fusicoccin.

Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the...

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Main Authors: Aducci, P, Ballio, A, Blein, J P, Fullone, M R, Rossignol, M, Scalla, R
Formato: Artigo
Idioma:Inglês
Publicado: 1988
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC282294/
https://ncbi.nlm.nih.gov/pubmed/2903497
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