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Identification of a functional mammalian spliceosome containing unspliced pre-mRNA.

Functional 60S spliceosomes were assembled under conditions that block the first step of the mRNA splicing reaction. This block was imposed by carrying out the splicing reaction in nuclear extracts preincubated in 2.5 mM EDTA. Preparative amounts of the spliceosomes were isolated by gel filtration c...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Abmayr, S M, Reed, R, Maniatis, T
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1988
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282155/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2971971/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.19.7216
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