Identification of a functional mammalian spliceosome containing unspliced pre-mRNA.
Functional 60S spliceosomes were assembled under conditions that block the first step of the mRNA splicing reaction. This block was imposed by carrying out the splicing reaction in nuclear extracts preincubated in 2.5 mM EDTA. Preparative amounts of the spliceosomes were isolated by gel filtration c...
Gardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1988
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282155/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2971971/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.19.7216 |
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