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Resolving Conformational and Rotameric Exchange in Spin-Labeled Proteins Using Saturation Recovery EPR

The function of many proteins involves equilibria between conformational substates, and to elucidate mechanisms of function it is essential to have experimental tools to detect the presence of conformational substates and to determine the time scale of exchange between them. Site-directed spin label...

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Bibliografiske detaljer
Main Authors: Bridges, Michael D., Hideg, Kálmán, Hubbell, Wayne L.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2821067/
https://ncbi.nlm.nih.gov/pubmed/20157634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00723-009-0079-2
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