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A mariner-Based Transposon System for In Vivo Random Mutagenesis of Clostridium difficile

Understanding the molecular basis of Clostridium difficile infection is a prerequisite to the development of effective countermeasures. Although there are methods for constructing gene-specific mutants of C. difficile, currently there is no effective method for generating libraries of random mutants...

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Autores principales: Cartman, Stephen T., Minton, Nigel P.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2820977/
https://ncbi.nlm.nih.gov/pubmed/20023081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02525-09
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